›› 2017, Vol. 30 ›› Issue (9): 108-.

• 论文 • 上一篇    下一篇

基于荧光显微镜的显微视觉图像增强处理

常敏,刘天成   

  1. (上海理工大学 光电信息与计算机工程学院,上海 200093)
  • 出版日期:2017-09-15 发布日期:2017-11-03
  • 作者简介:常敏(1978-),女,博士,副教授。研究方向:精密仪器。刘天成(1991-),男,硕士研究生。研究方向:精密仪器。

Microscopic Visual Image Enhancement Processing based on Fluorescence Microscope

CHANG Min,LIU Tiancheng   

  1. (School of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology, Shanghai 200093,China)
  • Online:2017-09-15 Published:2017-11-03

摘要:

针对显微视觉景深较小,经常出现图像模糊、不清晰等问题,文中提出采取一种图像增强的处理方法。通过对显微采集原始图像进行灰度变换、直方图均衡化等处理方法从而快速准确地获得目标的图像特征。基于图像增强、目标细胞定位处理对荧光图像中信息进行分析,找寻目标位置,提高细胞定位精确度。根据图像清晰度评价函数显示,经由处理后的图像边界和细节部分清晰,有助于医学应用。

关键词: 显微视觉, 图像增强, 细胞定位, 图像清晰度评价函数

Abstract:

With a small microscopic visual depth of field, often appear problem such as image fuzzy, not clear, to take a method of image enhancement processing.Through microscopic to original image gray-scale transformation, histogram equalization processing methods so as to rapidly and accurately so as to obtain the image characteristics of the target.By image enhancement, target cells and localization of fluorescence image analysis in the information, to find the target location, improve cell positioning accuracy.According to image clarity evaluation function, the processed image is through the clear boundary and the details and help to medical applications.

Key words: micro vision;image enhancement;cellular localization;evaluation function for image definition

中图分类号: 

  • TP391.41