恒温PCR双通道荧光读数仪的系统设计

›› 2016, Vol. 29 ›› Issue (3): 110-.

恒温PCR双通道荧光读数仪的系统设计

单秋霞,李建杰,吴晓,杜与冲,袁旭军

(1.上海理工大学光电信息与计算机工程学院,上海 200093;2.上海科源电子科技有限公司,上海 201101)

出版日期:2016-03-15 发布日期:2016-03-18
作者简介:单秋霞(1990—),女,硕士研究生。研究方向:光学工程,生物电子。李建杰(1981—),男,博士,硕士生导师。研究方向:电磁场与微波技术。
基金资助:
上海市高校青年教师培养资助计划基金项目(ZZslg15015)

System Design of Constant Temperature Dual-channel PCR Fluorescent Reader

SHAN Qiuxia,LI Jianjie,WU Xiao,DU Yuchong,YUAN Xujun

(1.School of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;
2.Shanghai Cohere Electronics Technology Co.,Ltd,Shanghai 201101,China)

Online:2016-03-15 Published:2016-03-18

摘要/Abstract

摘要：

针对传统PCR检测仪在检测速度和精度上存在的问题,文中设计了恒温PCR双通道荧光读数仪系统,系统采用实时荧光核酸恒温扩增检测技术(SAT),大幅提高了扩增反应时间。然而考虑到SAT技术仍无法避免假阴性,文中在此基础上提出了双通道的光学系统设计,在检测样品阴阳性的同时对阴性样品进行实时监控。结果表明,该系统最快可在30 min内完成检测,可有效指示假阴性现象的出现,并具有检测速度快、精确度高、且稳定性好等优点。

关键词: PCR仪, 恒温扩增, 双通道荧光检测

Abstract:

A system of constant temperature dual-channel fluorescent reader is designed to improve the detection speed and accuracy of traditional ones.The system is based on the technology of real-time simultaneous amplification and testing (SAT),with which the time of amplification reaction can be greatly reduced.Since the technology of simultaneous amplification and testing still cannot avoid the false negative results,a dual-channel optical system is designed based on the technology to offer accurate detection of the characteristics of sample.Real-time monitoring of negative ones is also achieved.Experiments demonstrate that the system can indicate false negative results effectively within 30 minutes with high accuracy and good stability.

Key words: PCR instrument;isothermal amplification;dual-channel fluorescence detection

中图分类号:

TH776

单秋霞,李建杰,吴晓,杜与冲,袁旭军. 恒温PCR双通道荧光读数仪的系统设计[J]. , 2016, 29(3): 110-.

SHAN Qiuxia,LI Jianjie,WU Xiao,DU Yuchong,YUAN Xujun. System Design of Constant Temperature Dual-channel PCR Fluorescent Reader[J]. , 2016, 29(3): 110-.